Journal: Applied and Environmental Microbiology

Article Title: Engineering of Bacteriophages Y2:: dpoL1-C and Y2:: luxAB for Efficient Control and Rapid Detection of the Fire Blight Pathogen, Erwinia amylovora

doi: 10.1128/AEM.00341-17

Figure Lengend Snippet: Schematic representation of the construct generated by overlap PCR for cloning into pBluescript and subsequent homologous recombination with Y2 genomic DNA. Annealing regions of the primers are depicted by small black arrows, and the restriction sites of EcoRI and BamHI, as well as the RBS (thick vertical line), are indicated. The image is not drawn to scale.

Article Snippet: The sequence and the cloning vector pBluescript II SK(−) (Agilent Technologies, Santa Clara, CA) were prepared for ligation by restriction digestion with the HF endonucleases EcoRI and BamHI (New England BioLabs, Ipswich, MA).

Techniques: Construct, Generated, Clone Assay, Homologous Recombination

Journal: Molecular cell

Article Title: Dynamic Processing of Displacement Loops during Recombinational DNA Repair

doi: 10.1016/j.molcel.2019.01.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Myc (9E11) Santa Cruz Biotechnology sc-47694, lot F11 13; RRID: AB_627266 Mouse monoclonal anti-GAPDH Thermo Scientific MA5–15738, lot QG215126; RRID: AB_10977387 Chemicals, Peptides, and Recombinant Proteins Trioxsalen (TMP) Sigma-Aldrich Cat#T6137 lndole-3-acetic acid (IAA) sodium salt (Auxin) Sigma-Aldrich Cat#l5148 dCTP-alpha-P32 6000 uCi/mmol PerkinElmer Cat#BLU-513Z T4 DNA Ligase New England Biolabs Cat#M0202 EcoRI-HF New England Biolabs Cat#R3101 Hindlll-HF New England Biolabs Cat#R3104 Critical Commercial Assays LightCycler 480 SYBR Green I Master Roche Life Science Cat#04707516001 Ambion DecaPrime II DNA labeling kit Thermo Fisher Cat#AM1455 Experimental Models: Organisms/Strains Saccharomyces cerevisiae; Individual genotypes see Table S1 This study N/A Oligonucleotides Quantitative PCR primers, see Table S2 Eurofins Genomics N/A Hybridization oligonucleotides, see Table S2 Sigma-Aldrich N/A Software and Algorithms LightCycler 480 Software Roche Life Science Cat#04994884001 R ×64 3.2.0 R Project https://www.r-project.org/ Ape Plasmid Editor Wayne Davis https://biologylabs.utah.edu/jorgensen/wayned/ape/ ImageJ 1.51k Wayne Rasband https://imagej.nih.gov/ij/ Other Spectrolinker (with 365 nm UV bulbs) Spectroline XL-1500 Open in a separate window KEY RESOURCES TABLE Development of a physical assay for D-loop detection in cells Srs2, Mph1, and Sgs1-Top3-Rmi1 (STR) regulate D-loop levels in vivo Two distinct pathways (Srs2 and Mph1, STR) target different D-loop species Rdh54 delineates the two D-loop reversal pathways

Techniques: Recombinant, SYBR Green Assay, DNA Labeling, Real-time Polymerase Chain Reaction, Hybridization, Software, Plasmid Preparation

Journal: iScience

Article Title: Icaritin inhibits CDK2 expression and activity to interfere with tumor progression

doi: 10.1016/j.isci.2022.104991

Figure Lengend Snippet:

Article Snippet: BamH I EcoR I , NEB , #R3136V #R3101V.

Techniques: Recombinant, Control

Journal: Frontiers in Veterinary Science

Article Title: Radiographic and echocardiographic evaluation in rescued Korean raccoon dogs ( Nyctereutes procyonoides koreensis )

doi: 10.3389/fvets.2024.1361843

Figure Lengend Snippet: Method used to measure the vertebral heart scale (VHS). VHS was measured as per the previous measurement method used in dogs. The greatest long axis is the diameter from the center of ventral point of the carina at the trachea to the apex of the heart in the right lateral view, and the greatest short axis is the line perpendicular to the long axis. The long axis and short axis were extended toward the caudal side from the cranial edge of T4. The number of vertebrae covered in unit of 0.1 were added (A) . Vertebral left atrial score was also measured on the RL view from the ventral center of the carina to the most caudal side of the left atrium, where it meets the dorsal border of the caudal vena cava. The line equal to the length obtained above was extended from T4 toward the caudal side and expressed the number of vertebral body units to the nearest 0.1 vertebra (B) , Intercostal space (ICS), an indicator of heart size in canines and felines, was obtained by comparing the maximum diameter of the heart to the number of intercostal spaces on the lateral radiographic view. Sternal contact was measured by counting the number of intercostal spaces corresponding to the area in contact with the ventral silhouette of the heart and the sternum in the RL view (C) . The cardiothoracic ratio was measured on the VD view using the ratio of the thoracic width at the same level as the maximum width of the heart silhouette (D) .

Article Snippet: Thoracic radiographs were acquired using a digital radiographic machine (HF-525PLUS, ECORAY, SEOUL, KOREA).

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Radiographic and echocardiographic evaluation in rescued Korean raccoon dogs ( Nyctereutes procyonoides koreensis )

doi: 10.3389/fvets.2024.1361843

Figure Lengend Snippet: Mean, standard deviations, and 95% confidence interval of thoracic radiographic indices according to the sex and relationship between body weight.

Article Snippet: Thoracic radiographs were acquired using a digital radiographic machine (HF-525PLUS, ECORAY, SEOUL, KOREA).

Techniques: